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agarose gel electrophoresis
Agarose Gel Electrophoresis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+gels/Agarose%2C+Electrophoresis+Grade/pmc13263687-206-8-20
Average 99 stars, based on 1 article reviews
Agarose Gel Electrophoresis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+gels/Agarose%2C+Electrophoresis+Grade/pmc13263687-206-8-20
Average 99 stars, based on 1 article reviews
agarose gel electrophoresis - by Bioz Stars,
2026-10
99/100 stars
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Polymerase Chain Reaction:Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: All PCR reactions were performed with either OneTaq 2x Master Mix with Standard Buffer (New England Biolabs) or Q5 High-Fidelity 2x Master Mix (New England Biolabs). .. Correct fragment size of the PCR product was confirmed using an InvitrogenTM E-GelTM Power Snap Electrophoresis System with E-Gel EX 1–2 % Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: PCR reactions for E. coli samples were performed using Rapid Taq Master Mix (Vazyme) in a 15-μl reaction containing 7.5 μl Mix, 0.75 μl of each primer (10 μM), and 6 μl of diluted cell lysate supernatant. .. PCR amplicons were generated over 25 PCR cycles and were resolved by electrophoresis on 1%–1.5% Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: .. PCR reactions for DNA fragments were performed using KOD OneTM PCR Master Mix (TOYOBO) in a 50-μl reaction containing 25 μl Mix, 1.5 μl of each primer (10 μM), 1 μl DNA template, and 21 μL of ddH 2 O. PCR amplicons were generated over 30 cycles were resolved by electrophoresis on 1%–1.5% Article Title: Screening Antifungal and Antioxidant Activity of Macroalgae from SE Spain Highlights the Invader Rugulopteryx okamurae Article Snippet: PCR amplifications were performed using VWR Red Taq DNA polymerase Master Mix in a LifeExpress thermal cycler (BIOER, Hangzhou, China). .. For brown algae, the psbA gene was amplified using primers psbAF1 (5′-ATGACTGCTACTTTAGAAAGAC-3′) and psbAR2 (5′-TCATGCATWACTTCCATACCTA-3′) [ ] under a two-step cycling protocol: an initial denaturation (94 °C, 2 min), followed by 5 cycles (94 °C for 30 s, 45 °C for 30 s, and 72 °C for 1 min), and 35 cycles (94 °C for 39 s, 46.2 °C for 39 s, and 72 °C for 1 min), with a final extension at 72 °C for 7 min. Green algae were identified by amplification of the rbcL gene using primers rbcL B (5′-ATGTCACCACAAACAGAAACTAAAGCA-3′) and rbcL Q (5′-GATCTCCTTCCATACTTCACAAGC-3′) [ ], with cycling conditions of 94 °C for 3 min; 30 cycles of 94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1.5 min; and a final extension at 72 °C for 7 min. For red and brown algae, the COI gene was amplified using primers GazF2 (5′-CCAACCAYAAAGATATWGGTAC-3′) and GazR2 (5′-GGATGACCAAARAACCAAAA-3′) [ ], with an initial denaturation at 94 °C for 4 min, followed by 40 cycles (94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1 min) and a final extension at 72 °C for 7 min. PCR products were verified on 1% Article Title: Fermented ginseng by-products regulate calcium metabolism pathways, liver health, immune function and intestinal microbiota to improve eggshell quality and production performance in late-laying hens Article Snippet: The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primer pairs 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) in a T100 Thermal Cycler PCR system (Bio-Rad, USA). .. PCR products were extracted from 2% Article Title: Dataset of groundwater bacterial communities at varying distances from public cemeteries in surakarta, Indonesia Article Snippet: .. The PCR products were verified on 0.8 % Electrophoresis:Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: All PCR reactions were performed with either OneTaq 2x Master Mix with Standard Buffer (New England Biolabs) or Q5 High-Fidelity 2x Master Mix (New England Biolabs). .. Correct fragment size of the PCR product was confirmed using an InvitrogenTM E-GelTM Power Snap Electrophoresis System with E-Gel EX 1–2 % Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: PCR reactions for E. coli samples were performed using Rapid Taq Master Mix (Vazyme) in a 15-μl reaction containing 7.5 μl Mix, 0.75 μl of each primer (10 μM), and 6 μl of diluted cell lysate supernatant. .. PCR amplicons were generated over 25 PCR cycles and were resolved by electrophoresis on 1%–1.5% Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: .. PCR reactions for DNA fragments were performed using KOD OneTM PCR Master Mix (TOYOBO) in a 50-μl reaction containing 25 μl Mix, 1.5 μl of each primer (10 μM), 1 μl DNA template, and 21 μL of ddH 2 O. PCR amplicons were generated over 30 cycles were resolved by electrophoresis on 1%–1.5% Generated:Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: PCR reactions for E. coli samples were performed using Rapid Taq Master Mix (Vazyme) in a 15-μl reaction containing 7.5 μl Mix, 0.75 μl of each primer (10 μM), and 6 μl of diluted cell lysate supernatant. .. PCR amplicons were generated over 25 PCR cycles and were resolved by electrophoresis on 1%–1.5% Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: .. PCR reactions for DNA fragments were performed using KOD OneTM PCR Master Mix (TOYOBO) in a 50-μl reaction containing 25 μl Mix, 1.5 μl of each primer (10 μM), 1 μl DNA template, and 21 μL of ddH 2 O. PCR amplicons were generated over 30 cycles were resolved by electrophoresis on 1%–1.5% Staining:Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: PCR reactions for E. coli samples were performed using Rapid Taq Master Mix (Vazyme) in a 15-μl reaction containing 7.5 μl Mix, 0.75 μl of each primer (10 μM), and 6 μl of diluted cell lysate supernatant. .. PCR amplicons were generated over 25 PCR cycles and were resolved by electrophoresis on 1%–1.5% Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: .. PCR reactions for DNA fragments were performed using KOD OneTM PCR Master Mix (TOYOBO) in a 50-μl reaction containing 25 μl Mix, 1.5 μl of each primer (10 μM), 1 μl DNA template, and 21 μL of ddH 2 O. PCR amplicons were generated over 30 cycles were resolved by electrophoresis on 1%–1.5% other:Article Title: mRNA Delivery to the Retina restores REP1 Function in Choroideremia Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Algae:Article Title: Screening Antifungal and Antioxidant Activity of Macroalgae from SE Spain Highlights the Invader Rugulopteryx okamurae Article Snippet: PCR amplifications were performed using VWR Red Taq DNA polymerase Master Mix in a LifeExpress thermal cycler (BIOER, Hangzhou, China). .. For brown algae, the psbA gene was amplified using primers psbAF1 (5′-ATGACTGCTACTTTAGAAAGAC-3′) and psbAR2 (5′-TCATGCATWACTTCCATACCTA-3′) [ ] under a two-step cycling protocol: an initial denaturation (94 °C, 2 min), followed by 5 cycles (94 °C for 30 s, 45 °C for 30 s, and 72 °C for 1 min), and 35 cycles (94 °C for 39 s, 46.2 °C for 39 s, and 72 °C for 1 min), with a final extension at 72 °C for 7 min. Green algae were identified by amplification of the rbcL gene using primers rbcL B (5′-ATGTCACCACAAACAGAAACTAAAGCA-3′) and rbcL Q (5′-GATCTCCTTCCATACTTCACAAGC-3′) [ ], with cycling conditions of 94 °C for 3 min; 30 cycles of 94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1.5 min; and a final extension at 72 °C for 7 min. For red and brown algae, the COI gene was amplified using primers GazF2 (5′-CCAACCAYAAAGATATWGGTAC-3′) and GazR2 (5′-GGATGACCAAARAACCAAAA-3′) [ ], with an initial denaturation at 94 °C for 4 min, followed by 40 cycles (94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1 min) and a final extension at 72 °C for 7 min. PCR products were verified on 1% Amplification:Article Title: Screening Antifungal and Antioxidant Activity of Macroalgae from SE Spain Highlights the Invader Rugulopteryx okamurae Article Snippet: PCR amplifications were performed using VWR Red Taq DNA polymerase Master Mix in a LifeExpress thermal cycler (BIOER, Hangzhou, China). .. For brown algae, the psbA gene was amplified using primers psbAF1 (5′-ATGACTGCTACTTTAGAAAGAC-3′) and psbAR2 (5′-TCATGCATWACTTCCATACCTA-3′) [ ] under a two-step cycling protocol: an initial denaturation (94 °C, 2 min), followed by 5 cycles (94 °C for 30 s, 45 °C for 30 s, and 72 °C for 1 min), and 35 cycles (94 °C for 39 s, 46.2 °C for 39 s, and 72 °C for 1 min), with a final extension at 72 °C for 7 min. Green algae were identified by amplification of the rbcL gene using primers rbcL B (5′-ATGTCACCACAAACAGAAACTAAAGCA-3′) and rbcL Q (5′-GATCTCCTTCCATACTTCACAAGC-3′) [ ], with cycling conditions of 94 °C for 3 min; 30 cycles of 94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1.5 min; and a final extension at 72 °C for 7 min. For red and brown algae, the COI gene was amplified using primers GazF2 (5′-CCAACCAYAAAGATATWGGTAC-3′) and GazR2 (5′-GGATGACCAAARAACCAAAA-3′) [ ], with an initial denaturation at 94 °C for 4 min, followed by 40 cycles (94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1 min) and a final extension at 72 °C for 7 min. PCR products were verified on 1% Purification:Article Title: Screening Antifungal and Antioxidant Activity of Macroalgae from SE Spain Highlights the Invader Rugulopteryx okamurae Article Snippet: PCR amplifications were performed using VWR Red Taq DNA polymerase Master Mix in a LifeExpress thermal cycler (BIOER, Hangzhou, China). .. For brown algae, the psbA gene was amplified using primers psbAF1 (5′-ATGACTGCTACTTTAGAAAGAC-3′) and psbAR2 (5′-TCATGCATWACTTCCATACCTA-3′) [ ] under a two-step cycling protocol: an initial denaturation (94 °C, 2 min), followed by 5 cycles (94 °C for 30 s, 45 °C for 30 s, and 72 °C for 1 min), and 35 cycles (94 °C for 39 s, 46.2 °C for 39 s, and 72 °C for 1 min), with a final extension at 72 °C for 7 min. Green algae were identified by amplification of the rbcL gene using primers rbcL B (5′-ATGTCACCACAAACAGAAACTAAAGCA-3′) and rbcL Q (5′-GATCTCCTTCCATACTTCACAAGC-3′) [ ], with cycling conditions of 94 °C for 3 min; 30 cycles of 94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1.5 min; and a final extension at 72 °C for 7 min. For red and brown algae, the COI gene was amplified using primers GazF2 (5′-CCAACCAYAAAGATATWGGTAC-3′) and GazR2 (5′-GGATGACCAAARAACCAAAA-3′) [ ], with an initial denaturation at 94 °C for 4 min, followed by 40 cycles (94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1 min) and a final extension at 72 °C for 7 min. PCR products were verified on 1% Article Title: Fermented ginseng by-products regulate calcium metabolism pathways, liver health, immune function and intestinal microbiota to improve eggshell quality and production performance in late-laying hens Article Snippet: The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primer pairs 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) in a T100 Thermal Cycler PCR system (Bio-Rad, USA). .. PCR products were extracted from 2% Gel Extraction:Article Title: Screening Antifungal and Antioxidant Activity of Macroalgae from SE Spain Highlights the Invader Rugulopteryx okamurae Article Snippet: PCR amplifications were performed using VWR Red Taq DNA polymerase Master Mix in a LifeExpress thermal cycler (BIOER, Hangzhou, China). .. For brown algae, the psbA gene was amplified using primers psbAF1 (5′-ATGACTGCTACTTTAGAAAGAC-3′) and psbAR2 (5′-TCATGCATWACTTCCATACCTA-3′) [ ] under a two-step cycling protocol: an initial denaturation (94 °C, 2 min), followed by 5 cycles (94 °C for 30 s, 45 °C for 30 s, and 72 °C for 1 min), and 35 cycles (94 °C for 39 s, 46.2 °C for 39 s, and 72 °C for 1 min), with a final extension at 72 °C for 7 min. Green algae were identified by amplification of the rbcL gene using primers rbcL B (5′-ATGTCACCACAAACAGAAACTAAAGCA-3′) and rbcL Q (5′-GATCTCCTTCCATACTTCACAAGC-3′) [ ], with cycling conditions of 94 °C for 3 min; 30 cycles of 94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1.5 min; and a final extension at 72 °C for 7 min. For red and brown algae, the COI gene was amplified using primers GazF2 (5′-CCAACCAYAAAGATATWGGTAC-3′) and GazR2 (5′-GGATGACCAAARAACCAAAA-3′) [ ], with an initial denaturation at 94 °C for 4 min, followed by 40 cycles (94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1 min) and a final extension at 72 °C for 7 min. PCR products were verified on 1% Sequencing:Article Title: Screening Antifungal and Antioxidant Activity of Macroalgae from SE Spain Highlights the Invader Rugulopteryx okamurae Article Snippet: PCR amplifications were performed using VWR Red Taq DNA polymerase Master Mix in a LifeExpress thermal cycler (BIOER, Hangzhou, China). .. For brown algae, the psbA gene was amplified using primers psbAF1 (5′-ATGACTGCTACTTTAGAAAGAC-3′) and psbAR2 (5′-TCATGCATWACTTCCATACCTA-3′) [ ] under a two-step cycling protocol: an initial denaturation (94 °C, 2 min), followed by 5 cycles (94 °C for 30 s, 45 °C for 30 s, and 72 °C for 1 min), and 35 cycles (94 °C for 39 s, 46.2 °C for 39 s, and 72 °C for 1 min), with a final extension at 72 °C for 7 min. Green algae were identified by amplification of the rbcL gene using primers rbcL B (5′-ATGTCACCACAAACAGAAACTAAAGCA-3′) and rbcL Q (5′-GATCTCCTTCCATACTTCACAAGC-3′) [ ], with cycling conditions of 94 °C for 3 min; 30 cycles of 94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1.5 min; and a final extension at 72 °C for 7 min. For red and brown algae, the COI gene was amplified using primers GazF2 (5′-CCAACCAYAAAGATATWGGTAC-3′) and GazR2 (5′-GGATGACCAAARAACCAAAA-3′) [ ], with an initial denaturation at 94 °C for 4 min, followed by 40 cycles (94 °C for 1 min, 50 °C for 30 s, and 72 °C for 1 min) and a final extension at 72 °C for 7 min. PCR products were verified on 1% |